| 46. | Organogenesis is |
| a. | formation of callus tissue |
| b. | formation of root and shoots on callus tissue |
| c. | both (a) and (b) |
| d. | genesis of organs |
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Answer: (b).formation of root and shoots on callus tissue
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| 47. | Which of the following is used in the culture of regenerating protoplasts, single cells or very dilute cell suspensions? |
| a. | Nurse medium |
| b. | Nurse or feeder culture |
| c. | Both (a) and (b) |
| d. | None of these |
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Answer: (c).Both (a) and (b)
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| 48. | In a callus culture |
| a. | increasing level of cytokinin to a callus induces shoot formation and increasing level of auxin promote root formation |
| b. | increasing level of auxin to a callus induces shoot formation and increasing level of cytokinin promote root formation |
| c. | auxins and cytokinins are not required |
| d. | only auxin is required for root and shoot formation |
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Answer: (a).increasing level of cytokinin to a callus induces shoot formation and increasing level of auxin promote root formation
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| 49. | Protoplasts are the cells devoid of |
| a. | cell membrane |
| b. | cell wall |
| c. | both cell wall and cell membrane |
| d. | none of these |
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Answer: (b).cell wall
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| 50. | Which breeding method uses a chemical to strip the cell wall of plant cells of two sexually incompatible species? |
| a. | Mass selection |
| b. | Protoplast fusion |
| c. | Transformation |
| d. | Transpiration |
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Answer: (b).Protoplast fusion
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